PDF | The present investigation was carried out to evaluate the antioxidant nature of ethanolic extract of Terminalia arjuna bark (EETA) on. Bioactive Components and Antioxidant Properties of Terminalia arjuna L. Extracts . Shahid Chatha, Shahzad Ali, Department of Applied Chemistry. Barks extracts of four different trees (Azadirachta indica, Terminalia arjuna, Acacia Antioxidant activity (AA) was determined by measuring reducing power, .
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After 3 minutes of shaking, the absorbance of mixtures was measured with spectrophotometer U, Hitachi Instruments Inc,Tokyo, Japan at nm taken as peroxide contents. The arjuna extracts are found to be quite effective in preventing the oxidation.
It can be concluded from the results of the present study that the antioxidant activity of Terminalia arjuna L extracts varied considerably depending on the nature of extracting antioxidang as well as the nature of raw materials to be extracted. The antioxidant capacities of arjuna extracts were also evaluated by measuring their free radical scavenging abilities to 1,1 —diphenyl-2 picrylhydrazyl DPPH stable following the previously established method [ 14 ] with slight modifications.
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Reducing power of extract was determined according antioxidannt the procedure described previously [ 15 ]. The extraction was executed for 24 hours in an orbital shaker Gallenkamp, U.
Our results are comparable with those ranged from Research Article Open Access. The total flavonoids contents TFC were determined following the previously established spectrometric method [ terminzlia ]. Terminalia arjuna based phytochemicals are considered as one of the best heart tonic [ 7 ] therefore, it can be used on daily bases as tonic for healthy cardiovascular system.
Qntioxidant plant is very rich source of natural antioxidants due to which it has been extensively investigated for its uses in both Ayurvedic and Yunani systems of medicine [ 78 ]. The data regarding the total phenolic contents antixoidant from different extracts ranged from 6. Sample containing synthetic antioxidant, BHT was used as positive control.
The results suggested that extracts of Terminalia arjuna L. The antioxidants are molecules capable of decreasing or preventing the oxidation of substrate molecules. In the present study, it was observed that methanol was an efficient solvent and stem bark extracts of arjuna were antioxixant rich in antioxidant compounds. The upper layer of solution 5.
Briefly, 15 gram of ground material was taken in conical flask followed by the addition of mL of each solvent separately.
February 18, ; Published Date: The absorbance of the resulting solutions and the blank were recorded after 1 hour at room temperature. Our finding regarding flavonoids in the present study were comparable with the actuvity of other researchers [ tfrminalia ] worked on antioxidant activity of different plants including Acacia nilotica, Terminalia arjuna, Eugenia jambolana and Azadirachta indica using different solvent blends of ethanol, methanol and water.
Please leave a message, we will get back you shortly. Home Publications Conferences Register Contact. Our results in close agreement to the findings of some previous researcher regarding the yield of extract prepared in different blends of methanol solvent [ tsrminalia ]. Extraction yield was dependant on the chemical composition of the bark of tree, as the phenolic and flavonoids contents investigated in the bark extracts Terminalia arjuna from different areas [ 18 ]. The highest yield of methanolic stem bark extract as established in the present analysis revealed the greater efficiency of methanol as extracting solvent and that might be attributed to the high polarity of the methanol: The extraction yields of extracts antioxidanr ranged from 6.
Bioactive Components and Antioxidant Properties of Terminalia arjuna L. Extracts
The estimation of total phenolic contents TPC was carried out using Folin-Ciocalteu reagent method [ 12 ]. Where, A blank is the absorbance of the control reaction mixture, and A sample is the absorbance of the sample. Two different solvent systems aqueous ethanol water: The present results suggested that the methanolic extracts of arjuna are more active regarding the phenolic antioxidant compounds that could be exploited as potent source of natural antioxidants for functional food and pharmaceutics.
Therefore, this project was designed to investigate bioactive components phenolics and flavonoidsfree radical scavenging property and antioxidant activity of extracts from barks and leaves of arjuna plant indigenous to Pakistan.
Bioactive Components and Antioxidant Properties of Terminalia arjuna L.Extracts
Free radical scavenging activity was evaluated using stable DPPH free radical ? Percent inhibition of linoleic acid peroxidation by synthetic antioxidant BHT was taken as control and compared with arjuna extracts.
Antioxidant compounds terminate these chain reactions by removing free radical intermediates, and inhibit other oxidation reactions by being oxidized themselves [ 2 ]. TFC amounts were expressed as catechin equivalents per hundred antioxjdant of dry matter. Can’t read the image? This work was carried out to investigate the antioxidant activity and free radical scavenging capacity of leaves and stem bark extracts of Ajruna arjuna arjuna prepared acctivity aqueous ethanol water: It can be concluded from the results that arjuna extracts were good source of natural antioxidants.
Total phenolic contents of extracts was determined by Folin- Ciocalteu reagent due to its high specificity for antioxidanf polyphenolic compounds present in plant extracts as it did not interact with the other phytochemicals due to a complex formation between reducing species and phosphors-molybdic tungstate.
Antioxidant capacities of extracts of were also determined in terms of measurements of inhibition of oxidation in linoleic acid system [ 6 ]. Flavonoids arjunone, arjunolone, luteolingallic acid, ellagic acid, Oligomeric Proanthocyanidines OPCsphytosterols, calcium, magnesium, zinc and copper [ 10 ]. The mixture was kept at room temperature for 10 actkvity, and then 1. The percentage yield of the extracts was calculated by using the following formula.
The oxidation reactions can produce free radicals, which start chain reactions and subsequently can cause a large number of cardiovascular diseases in humans [ 1 ].
The bark and leave samples of Terminalia arjuna L. The earlier investigations in the relevant field had clearly demonstrated that methanol was a promising solvent for extraction of antioxidants from natural products [ 16 ]. The active components of Terminalia arjuna are tannins, triterpenoid saponin arjunic acid, arjunolic acid, arjungenin, and arjunglycosides [ 11 ]. J Food Process Technol 5: The sample containing butylated hydroxytoulene BHT was used as a positive control.
It was observed that the stem bark extract prepared in methanol, exhibited the highest yield All the experiments were conducted in three replicates and analyzed in triplicate.